| 质粒名称: | pcDNA3 Flag-humanTRAF2 (1087) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 66931 |
| 存储实验室: | Michael Karin |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TRAF2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Flag（插入N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3 Flag-humanTRAF2 (1087) was a gift from Michael Karin (Addgene plasmid # 66931 ; http://n2t.net/addgene:66931 ; RRID:Addgene_66931) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pcDNA3 Flag-humanTRAF2 (1087) 6927 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Mammalian Expression of TRAF2.
ACCESSION .
VERSION .
KEYWORDS pcDNA3 Flag-humanTRAF2 (1087)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6927)
TITLE Karin Lab plasmids
REFERENCE 2 (bases 1 to 6927)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6927
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(54..73)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 245..624
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 625..828
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 779..799
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 873..892
/label=T7
/note="T7 promoter, forward primer"
promoter 873..891
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 929..952
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(2490..2508)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2491..2508)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2528..2545)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2534..2758
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2804..3232
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2891..2910)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3101..3122
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3241..3261)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3246..3575
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3426..3561
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3488..3507
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3642..4436
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3696..3715)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4306..4325
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4610..4731
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4647..4666)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4701..4720
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4780..4796)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4780..4796)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4793..4815)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4804..4820
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4828..4858)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4873..4894
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5011..5028)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5182..5770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5262..5281)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5941..6801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6564..6583
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6802..6906)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
841 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
901 gcttggtacc gagctcggat ccaccatgga ctacaaggat gacgatgaca aggctgcagc
961 tagcgtgacc ccccctggct ccctggagtt gctacagccc ggcttctcca agaccctcct
1021 ggggaccaag ctggaagcca agtacctgtg ctccgcctgc agaaacgtcc tccgcaggcc
1081 cttccaggcg cagtgtggcc accggtactg ctccttctgc ctggccagca tcctcagctc
1141 tgggcctcag aactgtgctg cctgtgttca cgagggcata tatgaagaag gcatttctat
1201 tttagaaagc agttcggcct tcccagataa tgctgcccgc agggaggtgg agagcctgcc
1261 ggccgtctgt cccagtgatg gatgcacctg gaaggggacc ctgaaagaat acgagagctg
1321 ccacgaaggc cgctgcccgc tcatgctgac cgaatgtccc gcgtgcaaag gcctggtccg
1381 ccttggtgaa aaggagcgcc acctggagca cgagtgcccg gagagaagcc tgagctgccg
1441 gcattgccgg gcaccctgct gcggagcaga cgtgaaggcg caccacgagg tctgccccaa
1501 gttcccctta acttgtgacg gctgcggcaa gaagaagatc ccccgggaga agtttcagga
1561 ccacgtcaag acttgtggca agtgtcgagt cccttgcaga ttccacgcca tcggctgcct
1621 cgagacggta gagggtgaga aacagcagga gcacgaggtg cagtggctgc gggagcacct
1681 ggccatgcta ctgagctcgg tgctggaggc aaagcccctc ttgggagacc agagccacgc
1741 ggggtcagag ctcctgcaga ggtgcgagag cctggagaag aagacggcca cttttgagaa
1801 cattgtctgc gtcctgaacc gggaggtgga gagggtggcc atgactgccg aggcctgcag
1861 ccggcagcac cggctggacc aagacaagat tgaagccctg agtagcaagg tgcagcagct
1921 ggagaggagc attggcctca aggacctggc gatggctgac ttggagcaga aggtcttgga
1981 gatggaggca tccacctacg atggggtctt catctggaag atctcagact tcgccaggaa
2041 gcgccaggaa gctgtggctg gccgcatacc cgccatcttc tccccagcct tctacaccag
2101 caggtacggc tacaagatgt gtctgcgtat ctacctgaac ggcgacggca ccgggcgagg
2161 aacacacctg tccctcttct ttgtggtgat gaagggcccg aatgacgccc tgctgcggtg
2221 gcccttcaac cagaaggtga ccttaatgct gctcgaccag aataaccggg agcacgtgat
2281 tgacgccttc aggcccgacg tgacttcatc ctcctttcag aggccagtca acgacatgaa
2341 catcgcaagc ggctgccccc tcttctgccc cgtctccaag atggaggcaa agaattccta
2401 cgtgcgggac gatgccatct tcatcaaggc cattgtggac ctgacagggc tctaagcggc
2461 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
2521 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
2581 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
2641 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
2701 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
2761 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
2821 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
2881 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
2941 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
3001 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
3061 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
3121 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
3181 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
3241 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3301 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3421 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3481 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
3541 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
3601 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
3661 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
3721 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
3781 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
3841 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
3901 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
3961 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
4021 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
4081 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
4141 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
4201 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
4261 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
4321 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
4381 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
4441 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
4501 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
4561 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
4621 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
4681 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
4741 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
4801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
4861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4981 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
5041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
5101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
5161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
5221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
5281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
5341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
5401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
5461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
5521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
5581 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
5641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
5701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
5941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
6001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
6061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
6121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
6181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
6241 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
6301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
6361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
6421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
6481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
6541 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
6601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
6661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6901 tccgcgcaca tttccccgaa aagtgcc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pEBB HA RelA
- 下一篇：pEP4 E02S EN2L
